Ottis' paper in Metallomics

Years in the making !!

Conclusions

Treatment of melanoma with Cu, Zn or Fe thiomaltol complexes causes a strong apoptotic response in melanoma, with Cu by far most active.

Apoptosis increases with superstoichiometric additions of Cu at the same Htma dosing, which correlates with an increase in intracellular Cu. 

Cu/Htma treatment causes rapid morphological changes in melanoma cells.

Cu/Htma was shown to rapidly accumulate in lysomes preceding apoptosis, using EM and confocal microscopy.

Use of fluorescent dithiomaltol Httma shows its accumulation in extracellular membrane and lysosomes.

Cu/Htma treatment inhibits proteasomal activityconcurrent with cell death.